1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human IRAK1 (10478-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

WB result of IRAK1 antibody (10478-2-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRAK1 transfected HEK-293 cells.

Various lysates were subjected to SDS PAGE followed by western blot with 10478-2-AP (IRAK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10478-2-AP (IRAK1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10478-2-AP (IRAK1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP Result of anti-IRAK1 (IP:10478-2-AP, 4ug; Detection:10478-2-AP 1:600) with HeLa cells lysate 2800ug.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IRAK1 antibody (10478-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-Phalloidin (orange).

Jurkat cells were subjected to SDS PAGE followed by western blot with 18221-1-AP (IRAK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18221-1-AP (IRAK4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18221-1-AP (IRAK4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP Result of anti-IRAK4 (IP:18221-1-AP, 4ug; Detection:18221-1-AP 1:300) with K-562 cells lysate 4000ug.

WB result of IRAK1 antibody (66653-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRAK1 transfected HEK-293 cells.

HeLa cells were subjected to SDS PAGE followed by western blot with 66653-1-Ig (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66653-1-Ig (IRAK1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66653-1-Ig (IRAK1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®488 IRAK1 antibody (CL488-10478) at dilution of 1:100, CL555-Phalloidin (orange).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human IRAK1 (CL488-10478) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 IRAK1 antibody (CL647-10478) at dilution of 1:200.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IRAK1 (CL647-10478) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 IRAK1 antibody (CL555-10478) at dilution of 1:100, CL488-Phalloidin (green).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human IRAK1 (CL555-10478) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

WB result of IRAK1 antibody (66653-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRAK1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

HeLa cells were subjected to SDS PAGE followed by western blot with 66653-1-Ig (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66653-1-Ig (IRAK1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66653-1-Ig (IRAK1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 67180-1-Ig (IRAK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.